Night Regulation

Night means the time between the end of evening civil twilight and the beginning of morning civil twilight, as published in the Air Almanac, converted to local time.

14 CFR 1.1 · the regulation governs
Also defined in 1 more source

The time between the end of evening civil twilight and the beginning of morning civil twilight, as published in the American Air Almanac.

Guidance: Helicopter Flying Handbook, p. G-4